BOIREAU, W.; BOMBARD, S.; SARI, M.A.; POMPON, D. Bioengineering and characterization of DNA-protein assemblies floating on supported membranes BIOTECHNOL BIOENG V.77 NO.2 [JAN 20 2002] PP.225-231 D Pompon/CNRS/Ctr Genet Mol/Lab Ingn Prot Membranaires/F-91198 Gif Sur Yvette/FRANCE ___________________________________________________ A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus consti- tuting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra-assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self-organization and fluidity pro- perties and is of interest as a prototype to design new generations of DNA chip bio-sensors. (C) 2002 John Wiley Sons, Inc. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ RANDAL, J. Nanotechnology getting off the ground in cancer research J NAT CANCER INST V.93 NO.24 [DEC 19 2001] PP.1836-1838 +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ BORM, P.J.A. Particle toxicology: From coal mining to nanotechnology INHAL TOXICOL V.14 NO.3 [MAR 2002] PP.311-324 PJA Borm/Univ Dusseldorf/Dusseldorf Inst Environm Res/Particle Core/Pos- tfach 103751/D-40028 Dusseldorf/GERMANY ___________________________________________________ Particle research has been historically closely connected to industrial activities or materials, such as coal, asbestos, man-made mineral fibers, and more recently ambient particulate matter (PM). It is the purpose of this review to combine insights and developments in particle toxicology with the historical context of exposure and organizations sponsoring such research in Europe. In supporting research on particle-induced respiratory effects and mechanisms, research programs of the European Community on Steel and Coal (ECSC) have played a tremendous role. Current particle research in Europe is dominated by PM, and funded by the World Health Orga- nization (WHO), European Union Framework programs, and the Health Effects Institute (HEI). Differences between historical and current research in particle toxicology include the exposure concentrations, particle size, target populations, endpoints, and length of exposure. Inhaled particle effects are no longer confined to the lung, since particles are suggested to translocate to the blood while lung inflammation invokes systemic res- ponses. Finally, the particle size and concentrations have both been reduced about 100-fold from 2-5 mg/m(3) to 20-50 mg/m(3) and from 1-2 mum to 20-100 nm ( ultrafine) as domestic fuel burning has decreased and vehicle sources have increased and attention has moved from coal mining industry to general environment. There is, however, a further occupational link to nanotechnology, which continuously produces new materials in the ultrafine range. Although inhalation exposure is considered to be minimal in this technology, some particles are produced to be used for carrier purpose in medical applications. Based on our current knowledge of par- ticle toxicology, it is highly desirable that toxicology and technology are linked in this extremely rapid developing area, to learn more about potential risks and also to develop knowledge on the role of surface and size in particle toxicity. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ SERVICE, R.F. Nanotechnology - Nanowire fabricators earn their stripes SCIENCE V.295 NO.5557 [FEB 8 2002] PP.946-947 +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ NISHIKAWA, S.; HOMMA, K.; KOMORI, Y.; IWAKI, M.; WAZAWA, T.; IWANE, A.H.; SAITO, J.; IKEBE, R.; KATAYAMA, E.; YANAGIDA, T.; IKEBE, M. Class VI myosin moves processively along actin filaments backward with large steps BIOCHEM BIOPHYS RES COMMUN V.290 NO.1 [JAN 11 2002] PP.311-317 M Ikebe/Univ Massachusetts/Sch Med/Dept Physiol/55 Lake Ave N/Worcester/MA 01655 USA ___________________________________________________ Among a superfamily of myosin, class VI myosin moves actin filaments backwards. Here we show that myosin VI moves processively on actin fila- ments backwards with large (similar to36 nm) steps, nevertheless it has an extremely short neck domain. Myosin V also moves processively with large (similar to36 nm) steps and it is believed that myosin V strides along the actin helical repeat with its elongated neck domain that is critical for its processive movement with large steps. Myosin VI having a short neck cannot take this scenario. We found by electron microscopy that myosin VI cooperatively binds to an actin filament at similar to36 nm intervals in the presence of ATP, raising a hypothesis that the binding of myosin VI evokes ''hot spots'' on actin filaments that attract myosin heads. Myosin VI may step on these ''hot spots'' on actin filaments in every helical pitch, thus producing processive movement with 36 nm steps. (C) 2002 Else- vier Science. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ CHEN, X.; LIN, Y.M.; GILSON, M.K. The Binding Database: Overview and user's guide BIOPOLYMERS V.61 NO.2 [1901] PP.127-141 MK Gilson/Univ Maryland/Maryland Biotechnol Inst/Ctr Adv Res Biotechnol/- 9600 Gudelsky Dr/Rockville/MD 20850 USA ___________________________________________________ The large and growing body of experimental data on molecular binding is of enormous value in biology, pharmacology, and chemistry. Applications include the assignment of function to biomolecules, drug discovery, mole- cular modeling, and nanotechnology. However, binding data are difficult to find and access because they are available almost exclusively through scientific journals. BindingDB, a public, web-accessible database of measured binding affinities, is designed to address this problem. Bind- ingDB collects data for natural and modified biomolecules and for syn- thetic compounds, and provides detailed experimental information. Cur- rently, measurements by isothermal titration calorimetry are fully sup- ported; measurements by enzyme inhibition will soon be included as well. The web site allows data to be searched by a range of criteria, including binding thermodynamics, sequence homology, and chemical structure, sub- structure, and similarity. Experimentalists are encouraged to publicize their data by entering it into BindingDB via the online forms. Such data can be updated or revised by the depositor, if necessary, and will remain publicly accessible. User involvement and feedback are welcomed. (C) 2002 Wiley Periodicals, Inc. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ KRICKA, L.J.; FORTINA, P. Nanotechnology and applications: An all-language literature survey inclu- ding books and patents CLIN CHEM V.48 NO.4 [APR 2002] PP.662-665 LJ Kricka/Univ Penn/Med Ctr/Dept Pathol & Lab Med/3400 Spruce St/Philadel- phia/PA 19104 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ KUNITAKE, M.; UEMURA, S.; ITO, O.; FUJIWARA, K.; MURATA, Y.; KOMATSU, K. Structural analysis of C-60 trimers by direct observation with scanning tunneling microscopy ANGEW CHEM INT ED V.41 NO.6 [1902] PP.969+ M Kunitake/Kumamoto Univ/Fac Engn/Dept Appl Chem & Biochem/Kumamoto 8608555/JAPAN +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ MOLDOVAN, N.I.; FERRARI, M. Prospects for microtechnology and nanotechnology in bioengineering of replacement microvessels ARCH PATHOL LAB MED V.126 NO.3 [MAR 2002] PP.320-324 NI Moldovan/Heart & Lung Res Inst/Room 305A/473 W 12th Ave/Columbus/OH 43210 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ MOLDOVAN, N.I.; FERRARI, M. Prospects for microtechnology and nanotechnology in bioengineering of replacement microvessels ARCH PATHOL LAB MED V.126 NO.3 [MAR 2002] PP.320-324 NI Moldovan/Heart & Lung Res Inst/Room 305A/473 W 12th Ave/Columbus/OH 43210 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ MOLDOVAN, N.I.; FERRARI, M. Prospects for microtechnology and nanotechnology in bioengineering of replacement microvessels ARCH PATHOL LAB MED V.126 NO.3 [MAR 2002] PP.320-324 NI Moldovan/Heart & Lung Res Inst/Room 305A/473 W 12th Ave/Columbus/OH 43210 USA ___________________________________________________ ONCLUSION.-WHILE NOTABLE ACCOMPLISHMENTS HAVE BEEN IDENTIFIED IN THE FIELD OF TISSUE ENGINEERING OF LARGER VESSELS, REPORTS ON PURPOSEFUL ASSEMBLY OF MICROVASCULAR STRUCTURES WITH THE ABILITY TO BE TRANSFERRED IN VIVO BY IMPLANTATION ARE STILL SCARCE. 0003-9985 V. NO. [] PP. NI Moldovan/Heart & Lung Res Inst/Room 305A/473 W 12th Ave/Columbus/OH 43210 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ BRUMFIEL, G. Investigation into nanotechnology papers expands NATURE V.417 NO.6888 [MAY 30 2002] PP.473 +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ DEROSNAY, J. Bio-, info- and nanotechnology: biotics' expansion M S-MED SCI V.18 NO.4 [APR 2002] PP.489-491 J Derosnay/Dept Prospect & Evaluat/Cite Sci & Ind/F-75930 Paris 19/FRANCE +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ SEEMAN, N.C.; BELCHER, A.M. Emulating biology: Building nanostructures from the bottom up PROC NAT ACAD SCI USA V.99 NO. Suppl. 2 [APR 30 2002] PP.6451-6455 NC Seeman/NYU/Dept Chem/New York/NY 10003 USA ___________________________________________________ The biological approach to nanotechnology has produced self-assembled objects, arrays and devices; likewise, it has achieved the recognition of inorganic systems and the control of their growth. Can these approaches now be integrated to produce useful systems? +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ RONCO, C.; BOWRY, S.K.; BRENDOLAN, A.; CREPALDI, C.; SOFFIATI, G.; FORTU- NATO, A.; BORDONI, V.; GRANZIERO, A.; TORSELLO, G.; LAGRECA, G. Hemodialyzer: From macro-design to membrane nanostructure; the case of the FX-class of hemodialyzers KIDNEY INT V.61 NO. Suppl. 80 [MAY 2002] PP.S126-S142 C Ronco/Osped San Bortolo/Div Nefrol/Dept Nephrol/Via Rodolfi 36100/I- 36100 Vicenza/ITALY ___________________________________________________ Hemodialyzer: From macro-design to membrane nanostructure; the case of the FX-class of hemodialyzers. Very few innovations have characterized the different components of the hemodialyzers in the past 20 years. Most impro- vements have concerned membrane biocompatibility. In this article. we focus our attention on the most recent advances in hemodialyzer components from the macro design of the unit to the nanostructure of the membrane. For this purpose, we took as an example the FX class of hemodialyzers (FMC, Bad Homburg. Germany). The studied devices were chosen as an example repre- senting some of the most recent hemodialyzers and are well suited to des- cribe technical innovations occurring in the field of dialyzer technology. In vitro and in vivo studies were performed to characterize hemodynamic parameters of three models (1.4-1, 8, and 2.2 m(2)) and to determine mem- brane permeability, sieving coefficients, and solute clearances. The units were characterized by a relatively high resistance of the blood and dialysate compartments, leading to an increased internal filtration if compared with similar hemodialyzers of other series. Nevertheless. the flow distribution in both compartments was homoReneous and well balanced. This effect was obtained by the improved blood and dialysate ports design, the increased packing density of the fibers and a reduction of the inner diameter of the fibers from 200 to 180 mum. At the same time, the sieving coefficients for middle-large solutes such as 02 microglobulin and insulin were higher than those observed in standard high flux dialysers. The same effect was noted for the clearance values of these solutes. This was observed in the absence of significant albumin leakage. Theis results were obtained thanks to a new nano-controlled spinning technology applied to the fiber. The innermost layer of the membrane is in fact characterized by a homogeneous porosity, with increased number of pores of large dimension but a sharp cutoff of the membrane excluding albumin losses. In conclusion. new technologies and new diagnostic tools today allow for improvement in hemodialyzer design from its macro-componets to its nano-structure. The application of nanotechnology to hemodialysis will probably contribute to further developments in hemodialyzer manufacturing. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ DEFRANCESCHI, S.; KOUWENHOVEN, L. Nanotechnology - Electronics and the single atom NATURE V.417 NO.6890 [JUN 13 2002] PP.701-702 S Defranceschi/Delft Univ Technol/Dept Nanosci/ERATO Mesoscop Correlat Project/POB 5046/NL-2600 GA Delft/NETHERLANDS +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ TERRETTAZ, S.; ULRICH, W.P.; VOGEL, H.; HONG, Q.; DOVER, L.G.; LAKEY, J.H. Stable self-assembly of a protein engineering scaffold on gold surfaces PROTEIN SCI V.11 NO.8 [AUG 2002] PP.1917-1925 JH Lakey/Med Sch Newcastle Upon Tyne/Sch Biochem & Genet/Newcastle Upon Tyne NE2 4HH/Tyne & Wear/ENGLAND ___________________________________________________ The outer membrane protein OmpF from Escherichia coli is a member of a large family of beta-barrel membrane proteins. Some, like OmpF, are pore- -forming proteins whilse others are active transporters or enzymes. We have previously shown that the receptor-binding domain (R-domain) of the toxin colicin N binds with high affinity to OmpF reconstituted into tethered lipid bilayers on gold electrodes. The binding can be measured by surface plasmon resonance (SPR) and ion channel blockage (impedance spec- troscopy, IS). In this paper we report the use of a mutant OmpF-E183C in which a single cysteine had been introduced on a short periplasmic turn. OmpF-E183C binds directly to gold surfaces and creates high-density pro- tein layers by self-assembly from detergent solution. When the gold sur- face is pretreated with beta-mercaptoethanol and thiolipids are added after the protein immobilisation step, the protein is shown, by Fourier transform infrared spectroscopy (FTIR), to retain its beta-rich structure. Furthermore, we could also measure R-domain binding by SPR and IS, confir- ming the functional reconstitution of a self-assembled membrane protein monolayer at the gold surface. Because these beta-barrel proteins are recognized protein engineering scaffolds, the method provides a generic method for the simple self-assembly of protein interfaces from aqueous solution. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ PATZKE, G.R.; KRUMEICH, F.; NESPER, R. Oxidic nanotubes and nanorods - Anisotropic modules for a future nanote- chnology [REVIEW] ANGEW CHEM INT ED V.41 NO.14 [1902] PP.2446-2461 R Nesper/ETH Honggerberg/HCI/Inorgan Chem Lab/CH-8093 Zurich/SWITZERLAND +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ NULF, C.J.; COREY, D.R. DNA assembly using bis-peptide nucleic acids (BisPNAs) NUCL ACID RES V.30 NO.13 [JUL 1 2002] PP.2782-2789 DR Corey/Univ Texas/SW Med Ctr/Dept Pharmacol/5323 Harry Hines Blvd/Dallas/- TX 75390 USA ___________________________________________________ DNA nanostructures are ordered oligonucleotide arrangements that have applications for DNA computers, crystallography, diagnostics and material sciences. Peptide nucleic acid (PNA) is a DNA/RNA mimic that offers many advantages for hybridization, but its potential for application in the field of DNA nanotechnology has yet to be thoroughly examined. We report the synthesis and characterization of tethered PNA molecules (bisPNAs) designed to assemble two individual DNA molecules through Watson-Crick base pairing. The spacer regions linking the PNAs were varied in length and contained amino acids with different electrostatic properties. We observed that bisPNAs effectively assembled oligonucleotides that were either the exact length of the PNA or that contained overhanging regions that projected outwards. In contrast, DNA assembly was much less efficient if the oligonucleotides contained overhanging regions that projected inwards. Surprisingly, the length of the spacer region between the PNA sequences did not greatly affect the efficiency of DNA assembly. Reasons for inefficient assembly of inward projecting DNA oligonucleotides include non-sequence-specific intramolecular interactions between the overhanging region of the bisPNA and steric conflicts that complicate simultaneous binding of two inward projecting strands. These results suggest that bisPNA molecules can be used for self-assembling DNA nanostructures pro- vided that the arrangement of the hybridizing DNA oligonucleotides does not interfere with simultaneous hybridization to the bisPNA molecule. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ NIEMEYER, C.M. Nanotechnology: Tools for the biomolecular engineer SCIENCE V.297 NO.5578 [JUL 5 2002] PP.62-63 CM Niemeyer/Univ Dortmund/Dept Chem/D-44227 Dortmund/GERMANY +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ SPATZ, J.P. Nano- and micropatterning by organic - Inorganic templating of hierar- chical self-assembled structures ANGEW CHEM INT ED V.41 NO.18 [1902] PP.3359-3362 JP Spatz/Univ Heidelberg/Inst Phys Chem/INF 253/D-69120 Heidelberg/GERMANY +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ MATHUR, N. Nanotechnology - Beyond the silicon roadmap NATURE V.419 NO.6907 [OCT 10 2002] PP.573+ N Mathur/Univ Cambridge/Dept Mat Sci & Met/New Museums Site/Pembroke St/- Cambridge CB2 3QZ/ENGLAND +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ BOGUNIAKUBIK, K.; SUGISAKA, M. From molecular biology to nanotechnology and nanomedicine [REVIEW] BIOSYSTEMS V.65 NO.2-3 [MAR-MAY 2002] PP.123-138 K Boguniakubik/Oita Univ/Fac Engn/Dannoharu 700/Oita 8701192/JAPAN ___________________________________________________ Great progress in the development of molecular biology techniques has been seen since the discovery of the structure of deoxyribonucleic acid (DNA) and the implementation of a polymerase chain reaction (PCR) method. This started a new era of research on the structure of nucleic acids molecules, the development of new analytical tools, and DNA-based analyses. The latter included not only diagnostic procedures but also, for example, DNA- -based computational approaches. On the other hand, people have started to be more interested in mimicking real life, and modeling the structures and organisms that already exist in nature for the further evaluation and insight into their behavior and evolution. These factors, among others, have led to the description of artificial organelles or cells, and the construction of nanoscale devices. These nanomachines and nanoobjects might soon find a practical implementation, especially in the field of medical research and diagnostics. The paper presents some examples, illus- trating the progress in multidisciplinary research in the nanoscale area. It is focused especially on immunogenetics-related aspects and the wide usage of DNA molecules in various fields of science. In addition, some proposals for nanoparticles and nanoscale tools and their applications in medicine are reviewed and discussed. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ NISHIDA, S.; FUNABASHI, Y.; IKAI, A. Combination of AFM with an objective-type total internal reflection fluorescence microscope (TIRFM) for nanomanipulation of single cells ULTRAMICROSCOPY V.91 NO.1-4 [MAY 2002] PP.269-274 A Ikai/Tokyo Inst Technol/Biodynam Lab/Fac Biosci & Biotechnol/Grad Sch Biosci & Biotechnol/Midori Ku/4259 Nagatsuta Cho/Yokohama/Kanagawa 2268501/- JAPAN ___________________________________________________ A new instrument was constructed by combining an objective-type total internal reflection fluorescence microscope with an atomic force micros- cope (AFM). Our purpose of constructing such an instrument is to detect and confirm the result of cellular level manipulations made with the AFM part through the detection system of the highly sensitive fluorescence microscope part. In this combination, manipulations are now possible from the nanometer to the micrometer scales and the fluorescence detection system is sensitive enough even for localizing single molecules. In this paper, we applied the system as a precise intracellular injector (nanoplanter). Fluorescent beads were first chemically immobilized onto a ZnO whisker that was glued to an AFM tip and were injected into a living BALB/3T3 cell together with the whisker. It was demonstrated that the system could clearly show the result of injection, that is, the presence of a small number of fluorescent beads in the cell. (C) 2002 Elsevier Science B.V. All rights reserved. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ APPELL, D. Nanotechnology: Wired for success NATURE V.419 NO.6907 [OCT 10 2002] PP.553-555 +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ SANDHU, A.; MASUDA, H.; ORAL, A.; BENDING, S.J.; YAMADA, A.; KONAGAI, M. Room temperature scanning Hall probe microscopy using GaAs/AlGaAs and Bi micro-hall probes ULTRAMICROSCOPY V.91 NO.1-4 [MAY 2002] PP.97-101 A Sandhu/Tokai Univ/Dept Elect & Elect Engn/1117 Kitakaname/Kanagawa 2591292/JAPAN ___________________________________________________ A room temperature scanning Hall probe microscope system utilizing GaAs/- AlGaAs and bismuth micro-Hall probes was used for magnetic imaging of fer- romagnetic domain structures on the surfaces of crystalline thin film gar- nets and permanent magnets. The Bi micro-Hall probes had dimensions ran- ging between 0.25 and 2.8 mum(2) and were fabricated using a combination of optical lithography and focused ion beam milling. The use of bismuth was found to overcome surface depletion effects associated with semiconduc- ting micro-Hall probes. Our experiments demonstrated that Bi is a prac- tical choice of material for fabricating sub-micron sized Hall sensors. (C) 2002 Elsevier Science B.V. All rights reserved. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ ECKARDT, L.H.; NAUMANN, K.; PANKAU, W.M.; REIN, M.; SCHWEITZER, M.; WIN- DHAB, N.; VONKIEDROWSKI, G. DNA nanotechnology: Chemical copying of connectivity NATURE V.420 NO.6913 [NOV 21 2002] PP.286 LH Eckardt/Ruhr Univ Bochum/Lehrstuhl Bioorgan Chem/D-44780 Bochum/GERMANY +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ LII, C.Y.; STOBINSKI, L.; TOMASIK, P.; LIAO, C.D. Single-walled carbon nanotube - potato amylose complex CARBOHYD POLYM V.51 NO.1 [JAN 1 1903] PP.93-98 P Tomasik/Univ Agr/Dept Chem/Mickiewicz Ave 21/PL-31120 Krakow/POLAND ___________________________________________________ Single-walled carbon nanotubes are wetted in aqueous solution of pure potato amylose and separated into homogenous suspension of nanotubes and sediment of bundled nanotubes. Similarly microcrystalline and colloidal graphite is wetted in the amylose solution whereas C-60 fullerenes remained non-wetted. In the micro-Raman spectra of the amylose complexes with single-walled nanotubes, a relatively large shift of the omega(D) and omega(G) modes towards a higher wave number was observed. In such a spectra of complexes of microcrystalline and colloidal graphite correspon- ding shifts were much subtler. These changes in the spectra of nanotubes were accompanied by clear changes in the ratio of the integrated intensi- ties of both the modes. No such changes could be noted in the spectra of amylose complexes with graphite. Optical, SEM and AFM microscopic observa- tions of solidified complexes suggest that small nanotubes could be enve- loped by amylose helices. (C) 2003 Elsevier Science Ltd. All rights reser- ved. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ RUTLEDGE, C.O. Stories from a life of learning AMER J PHARM EDUC V.66 NO.3 [FAL 2002] PP.329-332 CO Rutledge/Purdue Univ/Sch Pharm & Pharmacal Sci/Heine Pharm Bldg/W Lafayette/IN 47907 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ JIANG, K.L.; LI, Q.Q.; FAN, S.S. Nanotechnology: Spinning continuous carbon nanotube yarns - Carbon nano- tubes weave their way into a range of imaginative macroscopic applications NATURE V.419 NO.6909 [OCT 24 2002] PP.801 KL Jiang/Tsing Hua Univ/Dept Phys/Beijing 100084/PEOPLES R CHINA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ RUOSLAHTI, E. Antiangiogenics meet nanotechnology CANCER CELL V.2 NO.2 [AUG 2002] PP.97-98 E Ruoslahti/Burnham Inst/La Jolla/CA 92037 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ QUINTANA, A.; RACZKA, E.; PIEHLER, L.; LEE, I.; MYC, A.; MAJOROS, I.; PATRI, A.K.; THOMAS, T.; MULE, J.; BAKER, J.R. Design and function of a dendrimer-based therapeutic nanodevice targeted to tumor cells through the folate receptor PHARMACEUT RES V.19 NO.9 [SEP 2002] PP.1310-1316 JR Baker/Univ Michigan/Sch Med/Dept Internal Med/Ctr Biol Nanotechnol/Ann Arbor/MI 48109 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ QUINTANA, A.; RACZKA, E.; PIEHLER, L.; LEE, I.; MYC, A.; MAJOROS, I.; PATRI, A.K.; THOMAS, T.; MULE, J.; BAKER, J.R. Design and function of a dendrimer-based therapeutic nanodevice targeted to tumor cells through the folate receptor PHARMACEUT RES V.19 NO.9 [SEP 2002] PP.1310-1316 JR Baker/Univ Michigan/Sch Med/Dept Internal Med/Ctr Biol Nanotechnol/Ann Arbor/MI 48109 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ QUINTANA, A.; RACZKA, E.; PIEHLER, L.; LEE, I.; MYC, A.; MAJOROS, I.; PATRI, A.K.; THOMAS, T.; MULE, J.; BAKER, J.R. Design and function of a dendrimer-based therapeutic nanodevice targeted to tumor cells through the folate receptor PHARMACEUT RES V.19 NO.9 [SEP 2002] PP.1310-1316 JR Baker/Univ Michigan/Sch Med/Dept Internal Med/Ctr Biol Nanotechnol/Ann Arbor/MI 48109 USA ___________________________________________________ ONCLUSIONS. THESE RESULTS DEMONSTRATE THE ABILITY TO DESIGN AND PRODUCE POLYMER-BASED NANODEVICES FOR THE INTRACELLULAR TARGETING OF DRUGS, IMA- GING AGENTS, AND OTHER MATERIALS. 0724-8741 V. NO. [] PP. JR Baker/Univ Michigan/Sch Med/Dept Internal Med/Ctr Biol Nanotechnol/Ann Arbor/MI 48109 USA +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ STOBINSKI, L.; TOMASIK, P.; LII, C.Y.; CHAN, H.H.; LIN, H.M.; LIU, H.L.; KAO, C.T.; LU, K.S. Single-walled carbon nanotube - amylopectin complexes CARBOHYD POLYM V.51 NO.3 [FEB 15 1903] PP.311-316 P Tomasik/Univ Agr/Dept Chem/Mickiewicz Ave 21/PL-31120 Krakow/POLAND ___________________________________________________ Single-walled carbon nanotubes (SWCNTs) are wetted in aqueous solution of pure potato and waxy corn amylopectins. Formation of weak sorption com- plexes of amylopectins on carbon nanotubes is postulated based on the micro-Raman spectra, differential scanning calorimetric and thermogravi- metric studies as well as scanning electron micrographs and atomic force microscope images. Under an influence of ultrasounds bundles of SWCNTs disintegrated much more readily in aqueous solution of waxy corn amylo- pectin. In the solid state resulting from evaporation of suspensions to dryness interactions of SWCNTs with waxy corn amylopectin were also stronger than with potatoamylopectin. (C) 2003 Elsevier Science Ltd. All rights reserved. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ MOLL, D.; HUBER, C.; SCHLEGEL, B.; PUM, D.; SLEYTR, U.B.; SARA, M. S-layer-streptavidin fusion proteins as template for nanopatterned mole- cular arrays PROC NAT ACAD SCI USA V.99 NO.23 [NOV 12 2002] PP.14646-14651 M Sara/Gregor Mendel Str 33/A-1180 Vienna/AUSTRIA ___________________________________________________ Biomolecular self-assembly can be used as a powerful tool for nanoscale engineering. In this paper, we describe the development of building blocks for nanobiotechnology, which are based on the fusion of streptavidin to a crystalline bacterial cell surface layer (S-layer) protein with the inhe- rent ability to self-assemble into a monomolecular protein lattice. The fusion proteins and streptavidin were produced independently in Escheri- chia coli, isolated, and mixed to refold and purify heterotetramers of 1:3 stoichiometry. Self-assembled chimeric S-layers could be formed in suspen- sion, on liposomes, on silicon wafers, and on accessory cell wall polymer containing cell wall fragments. The two-dimensional protein crystals dis- played streptavidin in defined repetitive spacing, and they were capable of binding D-biotin and biotinylated proteins. Therefore, the chimeric S- -layer can be used as a self-assembling nanopatterned molecular affinity matrix to arrange biotinylated compounds on a surface. In addition, it has application potential as a functional coat of liposomes. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++